DFCI Flow Cytometry Core Facility
Bio-Plex 200(Luminex)
About the Bio-Plex System
The Bio-Ples system is a flexible, easy-to-use multiplex analysis system that permits the simultaneous analysis of up to 100 different biomolecules (proteins, peptides, or nucleic acids) in a single microplate well. By Multiplexing with the Bio-Plex system, researchers can dramatically increase the amount of useful information from rare or volume-limited samples such as mouse or rat serum, and decipher complex interrelationships among proteins involved in signal transduction pathways. The microplate platform allows the automated analysis of 96-well plates with a throughput of more than 1800 assay points (for a 23-plex assay) in 30 minutes.
The Bio-Plex suspension array is built around three core technologies. The first is the family of fluorescently dyed microsphers, or beads from Luminex Corp. The second is a flow cytometer with two lasers and associated optics to measure biochemical reactions that occur on the surface of the microspheres, and the third is a high-speed digital signal processor to efficiently manage the fluorescent output. These technologies are brought together in the Bio-Plex workstation.
Applications
Protein Expression Profiling
- Cytokines, Chemokines, and Growth Factors
- Cardiac Markers
- Cancer Markers
- Cellular Signaling
- Endocrine
- Isotyping
- Metabolic Markers
- Neurobiology
- Matric Metalloproteinases
- Transcription factors/Nuclear receptors
Genomic Research
- Genotyping
- Gene Expression Profiling
Genetic Disease
- Cystic Fibrosis
- Cytochrome p450
Immunodiagnostics
- Allergy Testing
- Autoimmune Disease
- HLA Testing
- Infectious Disease
Make your own kit (customize)
For more information contact BioRad.
Please do not hesitate to sign up for training.
Ordering Information: Contact BioRad, for pricing and ordering.